Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays.

نویسندگان

  • Shinji Fukuda
  • Yukie Sasaki
  • Masato Seno
چکیده

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 74 12  شماره 

صفحات  -

تاریخ انتشار 2008